KMID : 1134820190480020282
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Journal of the Korean Society of Food Science and Nutrition 2019 Volume.48 No. 2 p.282 ~ p.289
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Screening for Substances with Synergistic Anti-Inflammatory Effects in Combination with Fermented Platycodon grandiflorum Extracts in LPS-Stimulated RAW264.7 Cells
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Song Hee-Won
Lee So-Yeon Han Eun-Hye Yu Heui-Jong Lim Mi-Kyung
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Abstract
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The root extracts of Platycodon grandiflorum (PG) have been widely used as a phlegm-relieving herb. Platycodin D, derived from the root of P. grandiflorum, is known as an effective ingredient and possesses a variety of pharmacological effects such as anti-asthma, anti-inflammation, anti-obesity, anti-cancer, antimicrobial and immunomodulatory activities. This study aimed to find substances that could have an additive or synergistic effect on anti-inflammatory activities when combined with fermented P. grandiflorum extracts (FPG) containing increased platycodin D contents due to fermentation. Nitric oxide (NO) assays were performed using 14 natural product extracts, 6 minerals, and 11 vitamins. Among them, four substances, namely tartary buckwheat extracts, angelica (root) complex extracts, soybean extracts, and vitamin C, were selected for combination with FPG. As a result, soybean extracts and vitamin C exhibited additive or synergistic anti-inflammatory effects in combinatorial treatment with FPG, whereas tartary buckwheat extracts and angelica complex extracts did not exert synergistic effects. The combinatorial treatment of 200 ¥ìg/mL of FPG with soybean extracts or vitamin C inhibited NO production in LPS-stimulated RAW264.7 cells by 85.5%, and 76.3%, respectively, compared to treatment with FPG alone. In addition, combinatorial treatment of FPG with tartary buckwheat extracts or angelica complex extracts did not affect cell viability, however combinatorial treatment of 1,600 ¥ìg/mL of FPG with soybean extracts or vitamin C reduced cell viability. Therefore, these findings may be used as a basis for developing products with enhanced anti-inflammatory functionality using fermented P. grandiflorum extracts.
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KEYWORD
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fermented Platycodon grandiflorum, platycodin D, synergistic effect, nitric oxide, anti-inflammation
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